PHARMACEUTICAL MICROBIOLOGY - PY - 304
1. Introduction to the Science of Microbiology:
- Historical development, contribution of great scientists and scope of microbiology.
2. Microbiology Taxonomy-
- Classification of Bacteria and Viruses
3. Identification of Microbes
- Working of different types of microscopes,electron microscopy,stains and types of staining techniques.
- Structure and Morphology of bacteria and viruses.
- Nutritional requirements, Cultivation and isolation of bacteria and viruses.
4. Microbial genetics and variation.
5. Control of microbes by physical and chemical methods
- a. Disinfection: factors influencing disinfection, dynamics of disinfection.
- b. Disinfectants and antiseptics, and their evaluation
- c. Sterilization: Different methods, Validation of sterilization methods and equipments.
6. Sterility testing of pharmaceutical products.
7. Infection and factors influencing infection, immunity, Primary and Secondary defensive mechanism of body, Microbial resistance, Interferon.
8. Microbial diseases:
- Brief outline of communicable diseases. Their causative agents, Mode of transmission and prevention – Chicken pox, Measles, influenza, Diphtheria, Whooping, cough, Tuberculosis, Poliomyelitis, Hepatitis, Cholera, Typhoid, Food poisoning, Helminthiasis, Malaria, Filariasis, Rabies, Trachoma, Tetanus, Syphilis, Syphilis, Gonorrhoea and AIDS.
- Microbial assay of antibiotics and vitamins.
- Food spoilage and Preservation of food.
- Sewage and Sewage disposal: Industrial Sewage, Sewage treatment methods, BOD, COD etc.
LIST OF PRACTICALS:
(All practicals are compulsory)
- Study the motility of the given sample of microorganism by hanging drop technique.
- Identify the given sample of organism by simple staining technique
- Identify the given sample of organism by Gram staining technique
- Identify the given sample of organism by negative staining.
- Identify the bacteria by performing IMViC test.
- Prepare various types of culture media (Nutrient broth, nutrient agar, fluid thioglycolate media etc)
- Prepare subculture of the given sample of microorganism by aseptic transfer technique.
- Evaluate the given sample of disinfectant by R.W. Coefficient test.
- Determine the sterility of the given sample by filtration method.
- Determine the sterility of the given sample by direct inoculation method.
- Evaluate the given sample of an antibiotic microbiologically by filter paper disc method.
- Evaluate the given sample of an antibiotic microbiologically by cup plate method.
- Assay the given sample of vitamin microbiologically.
BOOKS RECOMMENDED:
- W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific publications, Oxford London.
- Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
- Gilbert S.Banker and Christopher T. Rhodes: Modern Pharmaceutics.
- Remington’s Pharmaceutical Sciences.
- Pelczar and Reid: Microbiology.
- Dawson and Mirne: Immunological and Blood products.
- Rose: Industrial Microbiology.
- Prescot and Dunn: Industrial Microbiology.
- Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
- Cooper and Gunn’s: Tutorial Pharmacy
- Peppler: Microbial Technology.
- I.P., B.P., U.S.P.- latest editions
- Edward: Fundamentals of Microbiology
- N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
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